GFP antibody

Cat. No. GTX113617
Cat. No.  GTX113617
Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX113617
GTX113617 IHC-Wm Image

Immunohistochemical analysis (whole mount) of transgenic zebrafish embryo (fixed with ice-cold acetone), using GFP(GTX113617) antibody at 1:200 dilution.

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Anti-GFP antibody used in Western Blot (WB). GTX113617
GTX113617 WB Image

IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-GFP antibody used in Western Blot (WB). GTX113617
GTX113617 WB Image

GFP antibody detects GFP protein by western blot analysis.
A. 1 μg 293T whole cell extract
B. 1 μg 293T whole cell extract expressing GFP-tagged protein
C. 1 μg 293T whole cell extract expressing RFP-tagged protein
12% SDS-PAGE
GFP antibody (GTX113617) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-GFP antibody used in Western Blot (WB). GTX113617
GTX113617 WB Image

GFP antibody detects GFP protein by Western blot analysis. Non-transfected (-) and GFP-transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody (GTX113617) diluted at 1:10000.

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Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113617
GTX113617 ICC/IF Image

Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( GTX113617, 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.

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Anti-GFP antibody used in Immunoprecipitation (IP). GTX113617
GTX113617 IP Image

GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody (GTX113617).
The precipitated GFP was detected by GTX113617 diluted at 1:15000.

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Anti-GFP antibody used in Western Blot (WB). GTX113617
GTX113617 WB Image

The data was published in the journal J Exp Clin Cancer Res in 2019. PMID: 31311575

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Anti-GFP antibody used in Western Blot (WB). GTX113617
GTX113617 WB Image

The data was published in the journal Commun Biol in 2019. PMID: 31754649

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Anti-GFP antibody used in IHC (Paraffin sections) (IHC-P). GTX113617
GTX113617 IHC-P Image

The data was published in the journal EMBO Mol Med in 2014. PMID: 24448489

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Anti-GFP antibody used in Western Blot (WB). GTX113617
GTX113617 WB Image

The data was published in the journal Commun Biol in 2019. PMID: 31754649

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Anti-GFP antibody used in Western Blot (WB). GTX113617
GTX113617 WB Image

The data was published in the journal Commun Biol in 2019. PMID: 31754649

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Anti-GFP antibody used in Western Blot (WB). GTX113617
GTX113617 WB Image

The data was published in the journal J Exp Clin Cancer Res in 2019. PMID: 31311575

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Anti-GFP antibody used in Western Blot (WB). GTX113617
GTX113617 WB Image

The data was published in the journal Adv Sci (Weinh) in 2020. PMID: 32195080

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Anti-GFP antibody used in Western Blot and Immunoprecipitation (WB IP). GTX113617
GTX113617 WB IP Image

The data was published in the journal J Virol in 2016. PMID: 27489281

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Anti-GFP antibody used in Western Blot (WB). GTX113617
GTX113617 WB Image

The data was published in the journal Cell Death Dis in 2018.PMID: 29752474

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Anti-GFP antibody used in IHC (Frozen sections) (IHC-Fr). GTX113617
GTX113617 IHC-Fr Image

The data was published in the journal Nat Commun in 2018.PMID: 30310071

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Anti-GFP antibody used in Western Blot (WB). GTX113617
GTX113617 WB Image

The data was published in the journal J Biomed Sci in 2018.PMID: 29361943

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Anti-GFP antibody used in Western Blot (WB). GTX113617
GTX113617 WB Image

The data was published in the journal Cell Death Dis in 2018.PMID: 29752474

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Anti-GFP antibody used in Western Blot and Immunoprecipitation (WB IP). GTX113617
GTX113617 WB IP Image

The data was published in the journal Cell Death Dis in 2018.PMID: 29752474

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Anti-GFP antibody used in Western Blot and Immunoprecipitation (WB IP). GTX113617
GTX113617 WB IP Image

The data was published in the 2020 in Front Mol Neurosci. PMID: 32587503

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Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113617
Anti-GFP antibody used in Immunoprecipitation (IP). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in IHC (Paraffin sections) (IHC-P). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot and Immunoprecipitation (WB IP). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in IHC (Frozen sections) (IHC-Fr). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot (WB). GTX113617
Anti-GFP antibody used in Western Blot and Immunoprecipitation (WB IP). GTX113617
Anti-GFP antibody used in Western Blot and Immunoprecipitation (WB IP). GTX113617
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB ICC/IF IHC-P IHC-Fr IHC-Wm IP Dot ELISA IHC
  • Reactivity
    Species independent
Summary
GFP antibody recognizes the green fluorescent protein (GFP), a fluorophore in widespread use in biomedical research. GFP fluorescence allows real-time study of physiological processes involving fusion proteins or GFP-expressing cells. This antibody also detects other GFP variants, including EGFP and YFP, but not red fluorescent protein (RFP).
See all GFP products